Imaging Bascis

多光子｜原理｜双光子显微镜的原理和应用 多光子｜原理｜三光子成像 Looking ever deeper - 上篇 ｜ 中篇 多光子｜原理｜深层、高速多光子在体成像技术 ｜ 问题解答 ｜ 前期海报 多光子｜实验｜双光子钙成像数据处理和分析 - 上篇 ｜ 下篇 多光子｜应用｜多光子成像技术在医疗诊断中的应用 多光子｜应用｜活体动物树突棘成像 大视场｜技术｜大视野成像、大规模神经活动记录技术 超分辨｜技术｜超分辨光学成像技术简介 超分辨｜技术｜提高分辨率：消卷积（Deconvolution） 拉曼散射｜技术｜受激拉曼散射显微成像： 无标记生物成像新方法 DIC｜应用｜雕刻光影-微分干涉相衬（DIC）成像应用 钙指示剂｜原理｜钙离子成像的原理 上转换荧光材料｜技术｜上转换发光纳米材料在生物医学中的应用 科普｜脑机融合系统在生物医学影像中的应用 科普｜类器官的显微成像 科普｜3D类器官与活体动态深层成像 科普｜荧光材料技术之纳米材料 科普｜共聚焦光谱成像：多色成像 ｜ 科普｜植物组织细胞光谱成像 科普｜病理报告为什么这么慢？我从手术室切下来的肉，你们拿去做了什么？ 科普｜三光子成像：大脑深处的“勘探器” 科普｜关于分辨率 科普｜关于噪声 文章撰写｜如何规范回复审稿人 科研绘图｜用matlab绘制的绝美图案

Publication list

文献中文解读：

1. NP 2021｜多光子显微镜kHz高速成像技术
2. NM 2020｜应用于高速多光子显微镜的自适应激发光源
3. CR｜双光子显微系统的飞秒激光直接特异性调控细胞的钙内流通道

Books:

1. Book Chapter 1997｜Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy

Review of MPM:

1. NM 2005｜Deep tissue two-photon microscopy
2. NBT 2003｜Nonlinear magic: multiphoton microscopy in the biosciences
3. NM 2017｜In vivo imaging of neural activity
4. JO 2018｜Optical toolkits for in vivo deep tissue laser scanning microscopy: a primer
5. Optica 2019｜Advanced fluorescence microscopy for in vivo imaging of neuronal activity
6. JN 2019｜Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy of In Vivo Neuronal Activity
7. Optica 2020｜Three-photon neuronal imaging in deep mouse brain

History of MPM:

1. Science 1990｜Two-Photon Laser Scanning Fluorescence Microscopy
2. PNAS 1996｜Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy
3. NP 2013｜In vivo three-photon microscopy of subcortical structures within an intact mouse brain
4. NM 2017｜In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
5. NM 2018｜Three-photon imaging of mouse brain structure and function through the intact skull

New technologies related to 3PM:

1. NM 2021｜AO｜An adaptive optics module for deep tissue multiphoton imaging in vivo
2. NM 2021｜AO｜High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy
3. Optica 2021｜AO｜Deep tissue scattering compensation with three-photon F-SHARP
4. BOE 2018｜Bessel-beam｜Rapid volumetric imaging with Bessel-Beam three-photon microscopy
5. OL 2018｜Bessel-beam｜Three-photon fluorescence microscopy with an axially elongated Bessel focus
6. BOE 2019｜Remote Focusing｜Dual-plane 3-photon microscopy with remote focusing
7. Cell 2019｜Combine 2PM｜Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy
8. NM 2020｜Head-mounted ｜Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats
9. NM 2021｜Laser｜An adaptive excitation source for high-speed multiphoton microscopy
10. OL 2018｜Light-sheet Microscope｜Three-photon light-sheet fluorescence microscopy
11. SA 2021｜Multicolor｜Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain

Applications of 3PM:

1. NC 2019｜Rat V1｜Functional imaging of visual cortical layers and subplate in awake mice with optimized threephoton microscopy
2. CC 2022｜Subventricular Zone｜Intravital Imaging of the Murine Subventricular Zone with Three Photon Microscopy
3. NM 2021｜Spinal Cord｜An adaptive optics module for deep tissue multiphoton imaging in vivo
4. Optica 2016｜Spinal Cord｜In-Vivo Three-Photon Excited Fluorescence Microscopy of the Spinal Cord in the Awake, Locomoting Mouse
5. NM 2021｜White Matter｜High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy
6. NI 2022｜Lymph Node｜Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes
7. NM 2020｜Zebrafish｜Deep 3P imaging of the brain in intact adult zebrafish
8. eLife 2022｜Drosophila｜Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
9. bioRxiv 2022｜Organoid｜Label-free 3P imaging of intact human cerebral organoids
10. LSA 2018｜Chick Embryo｜Dual-color deep-tissue three-photon microscopy with a multiband infrared laser

Tools

Olympus｜如何使用ImageJ/Fiji查看和处理Olympus显微镜的图片 Olympus｜如何使用FV31S-SW V2.5查看和处理Olympus显微镜的图片 Olympus｜如何使用Olyvia V3.3查看和处理Olympus显微镜的图片 Olympus｜本实验室显微镜介绍：FVMPE-RS 多光子激光扫描显微镜 Olympus｜本实验室光片显微镜介绍：Alpha3 光片显微成像系统 科研显微图像 AI 解决方案 Imaris｜Imaris在神经领域的应用 焊接基本操作