Selected Publications

An adaptive excitation source for high-speed multiphoton microscopy

Bo Li, Chunyan Wu, Mengran Wang, Kriti Charan and Chris Xu

Nature Methods, 17, 163-166 (2020). https://doi.org/10.1038/s41592-019-0663-9

We describe an adaptive femtosecond excitation source that only illuminates the region of interest, which leads to a 30-fold reduction in the power requirement for two- or three-photon imaging of brain activity in awake mice for improved high-speed longitudinal neuroimaging.

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

Cihang Kong, Christian Pilger, Henning Hachmeister, Xiaoming Wei, Tom H. Cheung, Cora S.W. Lai, Nikki P. Lee, Kevin. K. Tsia, Kenneth K. Y. Wong, Thomas Huser

Light: Science & Applications, 9(1), 1-12 (2020). https://doi.org/10.1038/s41377-020-0259-2

We present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability (improved by 50 dB), timing jitter (24.3 fs), average power fluctuation (<0.5%), modulation depth (>20 dB) and pulse width variation (<1.8%) over an extended wavenumber range (2700–3550 cm^−1), for high-contrast, fast CRS imaging without complicated noise reduction via balanced detection schemes.

Parallelized volumetric fluorescence microscopy with reconfigurable coded incoherent light-sheet array

Yu-Xuan Ren^#, Jianglai Wu^#, Queenie T. K. Lai^#, Hei Ming Lai , Dickson M. D. Siu , Wutian Wu , Kenneth K. Y. Wong and Kevin K. Tsia^*

Light: Science & Applications, 9(8), 2047-7538 (2020). https://doi.org/10.1038/s41377-020-0245-8

We hereby develop a novel fluorescence imaging approach, called coded light-sheet array microscopy (CLAM), which allows complete parallelized 3D imaging without mechanical scanning. The flexible implementation of CLAM regarding both hardware and software ensures compatibility with any light-sheet imaging modality and could thus be instrumental in a multitude of areas in biological research.

Full Publication list

2024

Liyun Lin, Jiaxin Liu, Zhengyuan Pan, Wen Pang, Xinyan Jiang, Man Lei, Jucai Gao, Yujie Xiao, Bo Li, Fang Hu, Zhouzhou Bao, Xunbin Wei, Wenbo Wu, Bobo Gu, "General Post-Regulation Strategy of AIEgens’ Photophysical Properties for Intravital Two-Photon Fluorescence Imaging," Advanced Science, 2024, 11, 2404792. https://doi.org/10.1002/advs.202404792
Yu-Xuan Ren, Johannes Frueh*, Zhisen Zhang, Sven Rutkowski, Yi Zhou, Huade Mao, Cihang Kong, Sergei I. Tverdokhlebov, Wen Liu, Kenneth K. Y. Wong*, and Bo Li*, "Topologically protected optical pulling force on synthetic particles through photonic nanojet," Nanophotonics, vol. 13, no. 2, 2024, pp. 239-249. https://doi.org/10.1515/nanoph-2023-0740
Peng Deng, Shoupei Liu, Yaoguang Zhao, Xinxin Zhang, Yufei Kong, Linlin Liu, Yujie Xiao, Shasha Yang, Jiahao Hu, Jixiong Su, Ang Xuan, Jinhong Xu, Huijuan Li, Xiaoman Su, Jingchuan Wu, Yu Mu, Zhicheng Shao*, Cihang Kong*, Bo Li* "Long-working-distance high-collection-efficiency three-photon microscopy for in vivo long-term imaging of zebrafish and organoids," iScience, vol. 27, no. 8, 2024, 110554. https://doi.org/10.1016/j.isci.2024.110554
李博，邓鹏，赵耀光，蒋玉立，孔慈航，“一种多光子显微镜的信号光收集装置及多光子显微成像系统”，中国专利（发明专利），202410476307.2，2024

2023

Yujie Xiao, Peng Deng, Yaoguang Zhao, Shasha Yang, Bo Li, "Three-photon excited fluorescence imaging in neuroscience: From principles to applications," Frontiers in Neuroscience, 2023, 17:1085682. https://doi.org/10.3389/fnins.2023.1085682
Bo Li, Lingjie Kong, Kiryl Piatkevich and Hod Dana, "Editorial: Large-scale Recording of Neuronal Activity at High Spatiotemporal Resolutions and Applications in Neuroscience," Frontiers in Neuroscience, 2023, 17:1202207. https://doi.org/10.3389/fnins.2023.1202207
任煜轩，孔慈航，李博 "光子喷流诱导后向作用力研究进展," 福建师范大学学报（自然科学版）, 2023, 39(6), 1-8. DOI:10.12046/j.issn.1000-5277.2023.06.001

2022

Weiwei Xue, Bo Li, Huihui Liu, Yujie Xiao, Bo Li, Lei Ren, Huijuan Li, Zhicheng Sha, "Generation of Dorsoventral Human Spinal Cord Organoids via Functionalizing Composite Scaffold for Drug Testing," iScience, 2022, 4, 39-43. https://doi.org/10.1016/j.isci.2022.105898
Yujie Xiao, Biqing Xue, Shasha Yang and Bo Li, "多光子成像技术：解析大脑深部的利剑," 科学, 2022, 4, 39-43. https://mall.cnki.net/magazine/magadetail/KXZZ202204.htm
Nan Zhang, Ruihong Dai, Weiqi Jiang, Yafei Meng, Bo Li and Fengqiu Wang, "950 nm Femtosecond Laser by Directly Frequency-doubling of a Thulium-doped Fiber Laser," IEEE Photonics Technology Letters, 2022, 34(9), 498-501. https://ieeexplore.ieee.org/document/9759336.DOI: 10.1109/LPT.2022.3168297.
Huiyun Yu, Leiyun Yang, Zhan Li, Feng Sun, Bo Li, Shengsong Guo, Yong-Fei Wang, Tong Zhou, Jian Hua, "In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene and its co‐expressed genes in Arabidopsis," Plant, Cell & Environment, 2022, 45(6), 1862-1875. https://doi.org/10.1111/pce.14293
任煜轩，孔慈航，李博，“一种结合光镊的受激拉曼显微成像装置”，中国专利（发明专利），202210658622.8，2022

2021

Fei Xia, Monique Gevers, Andreas Fognini, Aaron T. Mok, Bo Li, Najva Akbari, Iman Esmaeil Zadeh, Jessie Qin-Dregely, and Chris Xu, "Short-Wave Infrared Confocal Fluorescence Imaging of Deep Mouse Brain with a Superconducting Nanowire Single-Photon Detector," ACS Photonics, 2021, 8(9), 2800-2810. https://doi.org/10.1021/acsphotonics.1c01018
李博，赵耀光，任煜轩，肖宇婕，“一种具有高光子探测效率的多光子显微系统”，中国专利（实用新型专利），202123264431.3，2021

before 2020

Bo Li
1. B. Li, C. Wu, M. Wang, K. Charan, and C. Xu, "An adaptive excitation source for high speed multiphoton microscopy," Nature Methods, Vol. 17, pp. 163-166, 2020.
2. Chris Xu, Kriti Charan, Bo Li, Michael Buttolph. “Adaptive illumination apparatus, method, and applications” US Patent, US20200069233A1, 2020.
3. J. Yan, H. Yu, B. Li, A. Fan, J. Melkonian, X. Wang, T. Zhou, J. Hua, "Cell autonomous and non-autonomous functions of plant intracellular immune receptors in stomatal defense and apoplastic defense," PLoS pathogens, Vol. 15, pp. 1-25, 2019.
4. Y. Qin, B. Li, F. Xia, Y. Xia and C. Xu, "Multi-color background-free coherent anti-Stokes Raman scattering microscopy using a time-lens source," Opt. Express, vol. 26, pp. 34474-34483 2018.
5. F. Xia, C. Wu, D. Sinefeld, B. Li, Y. Qin and C. Xu, "In vivo label-free confocal imaging of the deep mouse brain with long-wavelength illumination," Bio. Opt. Express, vol. 9, pp. 6545-6555 2018.
6. M. Wang, C. Wu, D. Sinefeld, B. Li, F. Xia and C. Xu, "Comparing the effective attenuation lengths for long wavelength in vivo imaging of the mouse brain," Bio. Opt. Express, vol. 9, pp. 3534-3543 2018.
7. B. Li, M. Wang, K. Charan, and C. Xu, "Investigation of the long wavelength limit of soliton self-frequency shift in a silica fiber," Opt. Express, vol. 26, pp. 19637-19647 2018.
8. K. Charan, B. Li, M. Wang, C. P. Lin and C. Xu, "Fiber-based tunable repetition rate source for deep tissue two-photon fluorescence microscopy," Bio. Opt. Express, vol. 9, pp. 2304-2311 2018.
9. M. Li, S. Sun, B. Li, H. Asghari, Y. Deng, W. Li and N. Zhu, "Time-bandwidth compression of microwave signals," Opt. Express, vol. 26, pp. 990-999 2018.
10. F. Xia, D. Sinefeld, B. Li and C. Xu, Two-photon Shack–Hartmann wavefront sensor," Opt. Lett., vol. 42, pp. 1141-1144 2017.
11. B. Li, K. Charan, K. Wang, T. Rojo, D. Sinefeld, and C. Xu, "Nonresonant background suppression for coherent anti-Stokes Raman scattering microscopy using a multi-wavelength time-lens source," Opt. Express, vol. 24, pp. 26687-26695, 2016.
12. B. Li and J. Azaña, "Theory of Incoherent-Light Temporal Imaging Systems Based on a Temporal Pinhole," J. Lightwave Tech., vol. 34, pp. 2758-2773, 2016.
13. B. Li, S. Lou, and J. Azaña, "High-contrast linear optical pulse compression using a temporal hologram," Opt. Express, vol. 23, pp. 6833-6845, 2015.
14. B. Li and J. Azaña, "Temporal Imaging of Incoherent-Light Intensity Waveforms Based on a Gated Time-Lens System," Photonics J., IEEE, vol. 7, pp. 1-8, 2015.
15. B. Li, S. Lou, and J. Azaña, "Incoherent-light temporal imaging based on a temporal pinspeck," IEEE Photon. Tech. Lett., vol. 27, pp. 348-351, 2015.
16. B. Li, S. Lou, and J. Azaña, "Implementation of the photonic time-stretch concept using an incoherent pulsed light source," Appl. Opt., 54(10), pp. 2757-2761, 2015.
17. B. Li, S. Lou, and J. Azaña, "Novel Temporal Zone Plate Designs with Improved Energy Efficiency and Noise Performance," J. Lightwave Tech., 32(24), pp. 4201-4207, 2014.
18. B. Li and J. Azaña, "Incoherent-light temporal imaging of intensity waveforms," Opt. Photon. News, Year in optics, p. 33, 2014.
19. B. Li and J. Azaña, "Incoherent-light temporal stretching of high-speed intensity waveforms," Opt. Lett., 39(14), pp. 4243-4246, 2014.
20. S. Chang, S. Lou, B. Li, and B. Han, "Parametric modulation to extend the aperture of the time lens based on electro-optics modulation", Acta. Opt. Sin., pp. 65-70, 2014.
21. B. Li, M. Li, S. Lou, and J. Azaña, "Linear optical pulse compression based on temporal zone plates," Opt. Express, 21(14), pp. 16814-16830, 2013.
22. B. Li and S. Lou, "Elimination of Aberrations Due to High-Order Terms in Systems Based on Linear Time Lenses," J. Lightwave Tech., 31(13), pp. 2200-2206, 2013.
23. B. Li and S. Lou, "Time-frequency conversion, temporal filtering and temporal imaging using graded-index time-lenses," Opt. Lett., 37(19), pp. 3981-3983, 2012.
24. B. Li, S. Lou, and Z. Tan, "Two kinds of optical pulse compression approaches based on cross phase modulation," Acta. Phys. Sin. 61(19), 194203, 2012.
25. B. Li, Z. Tan, and X. Zhang, "Simulation and analysis of time lens using cross phase modulation and four-wave mixing," Acta. Phys. Sin. 61(1), 014203, 2012.
26. X. Zhang, Z. Tan, B. Li, and X. Sheng, "An optical-to-analog conversion technique based on temporal magnification," Optical Fiber & Electric Cable and Their Applications, 6, 26-29, 2011.
27. B. Li, Z. Tan, and X. Zhang, "Experiment and simulation of time lens using electro-optic phase modulation and cross phase modulation," Acta. Phys. Sin. 60(8), 084204, 2011.
Yuxuan Ren
1. Y.-X. Ren, X. Zeng, L. M. Zhou, C. Kong, H. Mao, C. W. Qiu, K. Tsia, K.K.Y. Wong, Photonic nanojet mediated back-action of dielectric microparticles, ACS Photonics, 7(6),1438-1490 (2020).
2. Y.-X. Ren, J. Wu, Q.T.K. Lai, H. M. Lai, D. Siu, W. T. Wu, K. K. Y. Wong, and K. K. Tsia, Parallelized volumetric fluorescence microscopy with reconfigurable coded incoherent light-sheet array, Light: Science & Applications, 9:8 (2020).
3. Y.-X. Ren, C. H. Kong, H. S. He, X. L. Zeng, K. K. Tsia, and K. K. Y. Wong, Encrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing, Appl. Phys. Express, 13, 032007 (2020).
4. S. H. Zhang, J. H. Zhou, and Y.-X. Ren, Ray optics analysis of optical forces on a microsphere in a (2+1) D Airy beam, OSA Continuum, 2(2),378-388 (2019).
5. Z.X. Fang, Y. Chen, Y.-X. Ren, L. Gong, R.D. Lu, A.Q. Zhang, H.Z. Zhao, and P. Wang, Interplay between topological phase and self-acceleration in vortex symmetric Airy beam, Optics Express, 26(6), 7324-7335 (2018).
6. Y.-X. Ren, T. S. Kelly, C.S. Zhang, H. Xu, and Z. Chen, Soliton-mediated orientational ordering of gold nanorods and birefringence in plasmonic suspensions, Optics Letters, 42(3), 627-630 (2017).
7. Y.-X. Ren, Z.X. Fang, L. Gong, K. Huang, Y. Chen, and R.D. Lu, Digital generation and control of Hermite-Gaussian modes with an amplitude digital micromirror device, Journal of Optics, 17, 125604(2015).
8. Y.-X. Ren, R.D. Lu, and L. Gong, Tailoring light with a Digital Micromirror Device, Annalen der Physik, 527(7-8), 447-470 (2015).
9. Y.-X. Ren, Z.X. Fang, L. Gong, K. Huang, Y. Chen, and R.-D. Lu, Dynamic generation of Ince-Gaussian modes with a digital micromirror device, J. Appl. Phys, 117(13), 133106 (2015).
10. Z.X. Fang, Y.-X. Ren, L. Gong, P Vaveliuk, Y. Chen, and R.D. Lu, Shaping symmetric Airy beam through binary amplitude modulation for ultralong needle focus, J. Appl. Phys., 118(20), 203102 (2015).
11. X.-Y. Ding, Y.-X. Ren, R.-D. Lu, Shaping super-Gaussian beam through amplitude reflection pattern using digital micromirror device. Science China Physics, Mechanics & Astronomy, 58(3), 034202 (2015).
12. X.-Y. Ding, Y.-X. Ren, L. Gong, Z. -X. Fang, and R.-D. Lu, Microscopic lithography with pixelate diffraction of digital micro-mirror device for micro-lens fabrication, Applied Optics, 53(24), 5307-5311 (2014).
13. L. Gong, Y.-X. Ren, G.-S. Xue, Q.-C. Wang, J.-H. Zhou, M.-C. Zhong, Z.-Q. Wang, and Y.-M. Li, Generation of non-diffracting Bessel beam using digital micromirror device, Applied Optics 52, 4566-4575 (2013).
14. Y.-X. Ren, J.-G.Wu, X.-W. Zhou, S.-J. Fu, Q. Sun, Z.-Q. Wang, and Y.-M. Li, Experimental generation of Laguerre-Gaussian beam using angular diffraction of binary phase plate, Acta Physica Sinica. 2010, 59(6):3930-3935
15. Y.-X. Ren, M. Li, K. Huang, J.-G. Wu, H.-F. Gao, Z.-Q. Wang, Y.-M. Li, Experimental generation of Laguerre-Gaussian beam using digital micromirror device, Applied Optics, 2010, 49(10):1838-1844.
16. Y.-X. Ren, J.-G. Wu, M. Chen, H. Li, Y.-M. Li, Stability of novel time-sharing dual optical tweezers using a rotating tilt glass plate, Chinese Physics Letters, 2010, 27(2): 028703.
17. Y.-X. Ren, J.-G. Wu, M.-C. Zhong, Y.-M. Li, Monte Carlo simulation of effective stiffness of time-sharing optical tweezers, Chinese Optics Letters, 2010, 8(2):170-172.
Cihang Kong
1. Kong, C, Pilger, C, Hachmeister, H, Wei, X, Cheung, T. H, Lai, C. S, ... & Huser, T. High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications, 9(1), 1-12.(2020).
2. Ren, Y. X, Zeng, X, Zhou, L. M, Kong, C, Mao, H, Qiu, C. W., ... & Wong, K. K. Photonic nanojet mediated backaction of dielectric microparticles. ACS Photonics. (2020).
3. He, H, Kong, C, Chan, K. Y, So, W. L, Fok, H. K, Ren, Y. X, ... & Wong, K. K. Resolution enhancement in an extended depth of field for volumetric two-photon microscopy. Optics Letters, 45(11), 3054-3057. (2020).
4. Ren, Y. X. Kong, C, He, H, Zeng, X, Tsia, K. K, & Wong, K. K. Encrypted wide-field two-photon microscopy with single-pixel detection and compressed sensing. Applied Physics Express, 13(3), 032007. (2020).
5. He, H, Kong, C, Tan, X. J, Chan, K. Y, Ren, Y. X, Tsia, K. K, & Wong, K. K. Depth-resolved volumetric two-photon microscopy based on dual Airy beam scanning. Optics letters, 44(21), 5238-5241. (2019).
6. Yu, Y, Kong, C, Li, B, Kang, J, Ren, Y. X, Luo, Z. C, & Wong, K. K. Behavioral similarity of dissipative solitons in an ultrafast fiber laser. Optics Letters, 44(19), 4813-4816. (2019).
7. Li, C, Kong, C, & Wong, K. K.High Energy Noise-Like Pulse Generation from a Mode-Locked Thulium-Doped Fiber Laser at 1.7 μm. IEEE Photonics Journal, 11(6), 1-6. (2019).
8. Wu, J, Mak, H. K, Chan, Y. K, Lin, C, Kong, C, Leung, C. K. S., & Shum, H. C. An in vitro pressure model towards studying the response of primary retinal ganglion cells to elevated hydrostatic pressures. Scientificreports,9(1),1-12.(2019).
9. Tan, X. J, Kong, C, Ren, Y. X, Lai, C. S, Tsia, K. K, & Wong, K. K. Volumetric two-photon microscopy with a non-diffracting Airy beam. Optics Letters, 44(2), 391-394. (2019).
10. Li, C, Shi, J, Gong, X, Kong, C, Luo, Z, Song, L, & Wong, K. K. 1.7 μm wavelength tunable gain-switched fiber laser and its application to spectroscopic photoacoustic imaging. Optics Letters,43(23),5849-5852.(2018).
11. Luo, Z. C, Kang, J. Q, Liu, M, Li, C, Kong, C. H, Yu, Y, & Wong, K. K. Optical rogue waves by random dissipative soliton buildup in a fiber laser. IEEE Photonics Technology Letters, 30(20), 1803-1806.(2018).
12. Kong, C, Wei, X, Kang, J, Tan, S, Tsia, K, & Wong, K. K. Ultra-broadband spatiotemporal sweeping device for high-speed optical imaging. Optics letters, 43(15), 3546-3549.(2018).
13. Kong, C, Pilger, C, Hachmeister, H, Wei, X, Cheung, T. H, Lai, C. S, ... & Wong, K. K. Compact fs ytterbium fiber laser at 1010 nm for biomedical applications. Biomedical Optics Express, 8(11), 4921-4932. (2017).
14. Kong, C, Wei, X, Tsia, K. K, & Wong, K. K. Ultrafast Green-Light Swept-Source Imaging Through Advanced Fiber-Optic Technologies. IEEE Journal of Selected Topics in Quantum Electronics, 24(3), 1-5. (2017).
15. Kang, J, Kong, C, Feng, P, Wei, X, Luo, Z. C, Lam, E. Y, & Wong, K. K. Broadband High-Energy All-Fiber Laser at 1.6 μm. IEEE Photonics Technology Letters, 30(4), 311-314.(2017).
16. Li, C, Wei, X, Kong, C, Tan, S, Chen, N, Kang, J, & Wong, K. K. Fiber chirped pulse amplification of a short wavelength mode-locked thulium-doped fiber laser. APL Photonics, 2(12), 121302. (2017).
17. Yang, T, Wei, ., Kong, C, Tan, S, Tsia, K. K, & Wong, K. K. An ultrafast wideband discretely swept fiber laser. IEEE Journal of Selected Topics in Quantum Electronics, 24(3), 1-5. (2017).
18. Wei, X, Kong, C, Sy, S., Ko, H, Tsia, K. K, & Wong, K. K. Ultrafast time-stretch imaging at 932 nm through a new highly-dispersive fiber. Biomedical optics express, 7(12), 5208-5217. (2016).
19. Wei, X, Kong, C, Samanta, G. K, Tsia, K. K, & Wong, K. K. Self-healing highly-chirped fiber laser at 1.0μm. OpticsExpress,24(24),27577-27586.(2016).